antibodies against il 23p19  (R&D Systems)

Journal: Journal of Cellular and Molecular Medicine

Article Title: IL‐23 plays a significant role in the augmentation of particulate matter‐mediated allergic airway inflammation

doi: 10.1111/jcmm.17475

Figure Lengend Snippet: Coculture of IL‐23 siRNA pre‐treated and PM/low‐dose Dp‐stimulated MLE12 cells with ILC2 or BMDCs. Experimental procedure (A). The protocol for sorting ILC2 from IL‐33 administered mice ( n = 8). For isolation of ILC2 from pooled mice, the population of ST2 + IL‐7R+ in lineage cells was sorted (B). For co‐cultivation, MLE‐12 cells were treated IL‐23p19 siRNA, and these MLE‐12 cells were stimulated by PM (0.1 μg/ml) or low dose of Dp (10 μg/ml) for overnight. After washing, cells were cocultured with ILC2. The gating plot and frequency of intracellular IL‐13 + IL‐5+ producing cells from isolated and expanded cells (C, D). For co‐cultivation with BMDCs, after treatment of IL‐23p19 siRNA in MLE‐12, PM or low dose of Dp was treated and after wash, then cocultured with BMDCs for 24 h. The gating plot and frequency of MHCII+CD86+ in CD11c+cells from BMDCs (E, F). PM: Particulate matter, IL‐23 si: IL‐23p19 siRNA. Statistically significant ( P < 0.05, P < 0.01) differences from Dp group are represented by *, differences from PM group are represented by #, and differences from Dp/PM group are represented by §. Statistical analysis followed by one‐way anova with Bonferroni's multiple comparisons test

Article Snippet: Mice were instilled PM or low dose of Dp (10 μg/mouse, Dermatophagoides pteronyssinus; Dp 1 1.88 μg/mg protein, PROLAGEN) with/without anti‐IL‐23p19 antibody (0.5 μg/mouse, R&D systems) by intranasal injection.

Techniques: Isolation

Journal: Journal of Cellular and Molecular Medicine

Article Title: IL‐23 plays a significant role in the augmentation of particulate matter‐mediated allergic airway inflammation

doi: 10.1111/jcmm.17475

Figure Lengend Snippet: Expression of IL‐23, IL‐23R and the effect of IL‐23 inhibition in PM‐ and low‐dose Dp‐stimulated MLE‐12 cells. IL‐23 production from cytosol and IL‐23R expression in PM or low dose of Dp‐treated MLE12 cells (A). Expression of IL‐23R was analysed using flow cytometry (B). IL‐23, GM‐CSF in cytosol and IL‐33 production in nucleus (C‐E), the gating plot including isotype (647‐conjugated anti‐goat IgG) and frequency of intracellular IL‐33 using flow cytometry (F‐G) in PM or low dose of Dp‐stimulated MLE12 cells with or without IL‐23p19 siRNA or NC (Normal Control) siRNA. Data were analysed using flow cytometry. PM: Particulate matter. IL‐23R: IL‐23 Receptor, IL‐23 si: IL‐23p19 siRNA. Statistically significant ( P < 0.05, P < 0.01, P < 0.001) differences from Dp group are represented by *, differences from PM group are represented by #, differences from Dp/PM group are represented by §. Statistical analysis followed by one‐way anova with Bonferroni's multiple comparisons test

Article Snippet: Mice were instilled PM or low dose of Dp (10 μg/mouse, Dermatophagoides pteronyssinus; Dp 1 1.88 μg/mg protein, PROLAGEN) with/without anti‐IL‐23p19 antibody (0.5 μg/mouse, R&D systems) by intranasal injection.

Techniques: Expressing, Inhibition, Flow Cytometry

Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

Article Title: Dendritic cells in irradiated mice trigger the functional plasticity and antitumor activity of adoptively transferred Tc17 cells via IL-12 signaling

doi: 10.1158/1078-0432.CCR-14-2294

Figure Lengend Snippet: Lymphodepletion induced IL-12 but not IL-23 enhances the antitumor activity of Tc17 cells in myeloablated mice. A, Levels of IL-12 and IL-23 produced by dendritic cells from spleens of mice receiving escalating doses of irradiation. One day post-irradiation, dendritic cells were sorted from splenocytes as CD11c+CD86hi from mice given 0, 5, or 9 Gy (+HSC) TBI, and dendritic cells were plated overnight in cell media. After 18 hours, supernatant from cultures was collected and analyzed via ELISA. Data are representative of 2 independent experiments (n=4 mice per group). B, B16F10 tumor bearing mice received 9 Gy TBI (+HSC) were either infused or not with pmel-1 Tc17 cells. Mice were injected 0 and 24 hours post-transfer with 100 μg neutralizing antibodies against either IL-12p35 or IL-23p19 or IgG isotype control. * = p<0.05, ** = p<0.01, *** = p<0.001. Statistics for Fig. 3A were determined by t-test and for Fig. 3B by a one way repeated measures ANOVA.

Article Snippet: Neutralizing antibodies against murine IL-12p35 (MMp35A1.6; eBioscience) or IL-23p19 (AF1619; R&D systems) were used as previously published [ 13 ] or as prescribed on the website.

Techniques: Activity Assay, Produced, Irradiation, Enzyme-linked Immunosorbent Assay, Injection, Control